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BlackBerry® Quenchers

Fluorous Affinity Purification of Oligonucleotides

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BlackBerry® Quenchers
Stable, long-wavelength quenchers of fluorescence

BlackBerry® Quencher 650 (BBQ-650®) is ideal for the quenching of long-wavelength reporter fluorophores, useful in various probe applications involving contact or FRET quenching.

Non-fluorescent, absorbs light at long wavelengths

• Useful absorbance between 550 and 750 nm.

• λmax varies from 650 to >690 nm in various oligonucleotides.

• Quenches long-wavelength fluorophores such as Cy3, TAMRA, Texas Red, Cy5, and Cy5.5.

Robust, stable to synthesis

• Ammonia stable for mild deprotection.

• Install at internal, 3', or 5' positions with standard solid-phase oligonucleotide synthesis.

Excellent contact-mode quencher

• Highly efficient quencher of FAM, Cy3, Texas Red, Cy5, and Cy 5.5 in contact quenching applications such as molecular beacons.

• Particularly high affinity for Cy dyes. Signal-to-background ratios of >90 and >88 for Cy5 and Cy5.5, respectively, were observed in molecular beacons.

Excellent FRET-mode quencher

• Excellent spectral overlap with long-wavelength fluorophores such as Texas Red, ROX, Cy5, and Cy 5.5, thus allowing efficient FRET quenching.

• Cy5.5 was quenched with 99% efficiency when separated from BBQ-650™ by ten base pairs (ca. 40 Å) in a non-contact hybrid probe.

 

BlackBerry® Quencher Products

Item
Catalog No.
Size/pack
Price (USD)
  
BL-2010
 (Add "-E" to part number for Expedite columns, "-A" for ABI-style crimp columns)
200 nmol columns (pkg of 4)
$75.00
   
1 µmol columns (pkg of 4)
$210.00
   
100 mg
$135.00
1 g
$1050.00
 
BL-2020
 (Add "-E" to part number for Expedite columns, "-A" for ABI-style crimp columns)
200 nmol columns (pkg of 4)
$80.00
   
1 µmol columns (pkg of 4)
$230.00
   
100 mg
$147.00
1 g
$1140.00
 
BL-2030
 (Add "-E" to part number for Expedite columns, "-A" for ABI-style crimp columns)
200 nmol columns (pkg of 4)
$75.00
   
1 µmol columns (pkg of 4)
$210.00
   
100 mg
$135.00
1 g
$1050.00
 
BL-1010
 
50 µmol
$150.00
   
100 µmol
$275.00
   
0.25 g
$650.00
       
BL-1020
  
50 µmol
$160.00
100 µmol
$280.00
   
0.25 g
$675.00
   
       
BL-1022
  
100 µmol
$280.00
0.25 g
$675.00
       
BL-1030
  
100 µmol
$390.00
   
0.25 g
$695.00
   
BL-3010
   
5 mg
$95.00
   
25 mg
$375.00
BL-2040
   
50 mg
$465.00
   
100 mg
$710.00
BL-3030
   
5 mg
$250.00
   
10 mg
$425.00

 

BlackBerry® Quenchers: Covered under US Patent #7,879,986, Berry & Associates, Inc.
"BlackBerry" is a registered trademark of Berry & Associates, Inc.

"BBQ-650" is a registered trademark of Berry & Associates, Inc.

See Terms, Warranty, Legal page for important information about BlackBerry products.
"Cy", "Cy3", "Cy5", and "Cy5.5" are trademarks of GE Healthcare. "Texas Red" is a registered trademark of Molecular Probes. "ROX", and "TAMRA" are trademarks of Applied Biosystems, Inc.

 



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