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Full Catalog (page 13)
Product Number Product Name Molecular Formula Cas. No.

FF 6120

6-Carboxyfluorescein-dipivalate
TEG azide

C39H44N4O11 

1254709-11-9 

Description

For a complete listing of our reagents for click chemistry, please see our Click-matesTM Collection page.

The purchase of these products for use in applications relating to copper catalyzed azide-alkyne cycloaddition chemistry (“Click Chemistry”) includes a limited, nontransferable license to intellectual property owned by TSRI to use this product solely for internal non-commercial research activities and specifically excludes clinical, therapeutic, or diagnostic use in humans or animals. Information regarding a license for commercial use in Click Chemistry may be obtained directly from The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla, CA 92037, or by contacting 858-784-8140 or click@scripps.edu.

Notes

Useful fluorescein azide from our Click-matesTM collection for fluorescein incorporation via Staudinger ligation or click chemistry.

Alternate Name(s):

6-FAM-dipivalate-TEG azide

FF 6130

6-TET-TEG azide

C29H24Cl4N4O9 

1333145-22-4 

Description

For a complete listing of our reagents for click chemistry, please see our Click-matesTM Collection page.

The purchase of these products for use in applications relating to copper catalyzed azide-alkyne cycloaddition chemistry (“Click Chemistry”) includes a limited, nontransferable license to intellectual property owned by TSRI to use this product solely for internal non-commercial research activities and specifically excludes clinical, therapeutic, or diagnostic use in humans or animals. Information regarding a license for commercial use in Click Chemistry may be obtained directly from The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla, CA 92037, or by contacting 858-784-8140 or click@scripps.edu.

Notes

Useful fluorescein azide from our Click-matesTM collection for tetrachloro-fluorescein incorporation via Staudinger ligation or click chemistry.

Alternate Name(s):

TET-PEG3 azide

6-Carboxy-2',4,7,7'-tetrachlorofluorescein-TEG azide

FF 6150

5-Carboxy-TET

C21H8Cl4O7 

155911-13-0 

Alternate Name(s):

2',4,7,7'-Tetrachlorofluorescein-5-carboxylic acid

FF 6155

5-Carboxy-TET dipivalate N-
hydroxysuccinimide ester

C35H27Cl4NO11 

None Assigned 

FF 6160

6-Carboxy-TET

C21H8Cl4O7 

155911-14-1 

Alternate Name(s):

2',4,7,7'-Tetrachlorofluorescein-6-carboxylic acid

FL 1000

FDMT-N6-Bz-dA
CEP

C57H55F17N7O7P 

865758-44-7 and 865758-49-2 

Description

Learn about fluorous affinity purification of oligonucleotides here. Download a brief overview here.

Notes

Fluorous-tagged nucleosides are useful for the purification of oligonucleotides. Use with Fluoro-Pak™ Columns (FP 7210 or FP 7220) and Loading Buffer (LB 7100 ).

Alternate Name(s):

5'-O-FDMT-N6-benzoyl-2'-deoxyadenosine CEP

N6-Benzoyl-3'-O-[(diisopropylamino)(2-cyanoethoxy)phosphino]-5'-O-[4,4'-dimethoxy-4"-(1H,1H,2H,2H-perfluorodecyl)trityl]-2'-deoxyadenosine

FL 1100

FDMT-N4-Bz-dC
CEP

C56H55F17N5O8P 

865758-45-8 and 865758-50-5 

Description

Learn about fluorous affinity purification of oligonucleotides here. Download a brief overview here.

Notes

Fluorous-tagged nucleosides are useful for the purification of oligonucleotides. Use with Fluoro-Pak™ Columns (FP 7210 or FP 7220) and Loading Buffer (LB 7100 ).

Alternate Name(s):

5'-O-FDMT-N4-benzoyl-2'-deoxycytidine CEP

N4-Benzoyl-3'-O-[(diisopropylamino)(2-cyanoethoxy)phosphino]-5'-O-[4,4'-dimethoxy-4"-(1H,1H,2H,2H-perfluorodecyl)trityl]-2'-deoxycytidine

FL 1200

FDMT-N2-iBu-dG
CEP

C54H57F17N7O8P 

865758-46-9 and 865758-51-6 

Description

Learn about fluorous affinity purification of oligonucleotides here. Download a brief overview here.

Notes

Fluorous-tagged nucleosides are useful for the purification of oligonucleotides. Use with Fluoro-Pak™ Columns (FP 7210 or FP 7220) and Loading Buffer (LB 7100 ).

Alternate Name(s):

5'-O-FDMT-N2-isobutyryl-2'-deoxyguanosine CEP

3'-O-[(Diisopropylamino)(2-cyanoethoxy)phosphino]-5'-O-[4,4'-dimethoxy-4"-(1H,1H,2H,2H-perfluorodecyl)trityl]-N2-isobutyryl-2'-deoxyguanosine

FL 1220

FMMT-N2-iBu-dG
CEP

C53H55F17O7P 

None Assigned 

Description

Download Product Information. Fluorous-tagged phosphoramidites are useful for the purification of oligonucleotides. Use with Fluoro-Pak™ Columns (FP 7210 or FP 7220) and Loading Buffer (LB 7100 ); see "Fluorous Purification of Oligonucleotides", which is included in with your purchase or may be downloaded.

Learn about fluorous affinity purification of oligonucleotides here, or download a brief overview here.

Notes

This monomer bears a fluorous-tagged monomethoxytrityl (FMMT) group rather than a fluorous-tagged dimethoxytrityl (FDMT) group, and is for applications where a more robust 5-terminal purification tag is needed.

Alternate Name(s):

3'-O-[(Diisopropylamino)(2-cyanoethoxy)phosphino]-5'-O-[4-methoxy-4-(1H,1H,2H,2H-perfluorodecyl)trityl]-N2-isobutyryl-2'-deoxyguanosine

FL 1300

FDMT-T CEP

C50H52F17N4O8P 

902456-09-1 

Description

Learn about fluorous affinity purification of oligonucleotides here. Download a brief overview here.

Notes

Fluorous-tagged nucleosides are useful for the purification of oligonucleotides. Use with Fluoro-Pak™ Columns (FP 7210 or FP 7220) and Loading Buffer (LB 7100 ).

Alternate Name(s):

5'-O-FDMT-thymidine CEP

3'-O-[(Diisopropylamino)(2-cyanoethoxy)phosphino]-5'-O-[4,4'-dimethoxy-4"-(1H,1H,2H,2H-perfluorodecyl)trityl]thymidine

FL 1360

F-CPR-II CEP

C49H54F17N2O9P 

1027512-47-5 

Description

Download Product Information. Fluorous Chemical Phosphorylation Reagent II (F-CPR-II CEP) allows a combination of fluorous affinity purification with concomitant 5'-phosphorylation, which is especially attractive when synthesizing longmers that will be used in ligase reactions. Further, if a 5'-phosphate is tolerated in a particular application, F-CPR-II may be used as a common handle for fluorous purification, obviating the need to choose a different fluorous phosphoramidite for each different 5'-terminal nucleotide. F-CPR-II CEP is used in the same way as CPR-II (Guzaev, A., et al., Tetrahedron 1995, 51, 9375-9384; US Patent 5,959,090, assignee Glen Research), i.e., a 6 minute coupling with no capping. Use with Fluoro-Pak™ Columns (FP 7210 or FP 7220) and Loading Buffer (LB 7100); see "Fluorous Purification of Oligonucleotides". The final retro-aldol cleavage to afford the free 5'-phosphorylated oligonucleotide is achieved in the same fashion as found for CPR-II.

Notes

For the chemical phosphorylation of oligonucleotides with concomitant fluorous affinity purification. Learn about fluorous affinity purification of oligonucleotides here or download a brief overview here.

Alternate Name(s):

Fluorous chemical phosphorylation reagent II

2,2'-Bis(ethoxycarbonyl)-1-O-[(diisopropylamino)(2-cyanoethoxy)phosphino]-3-O-[4,4'-dimethoxy-4''-(1H,1H,2H,2H-perfluorodecyl)trityl]-1,3-propanediol

FL 1400

FDMT-On purification modifier

C44H48F17N2O5P 

1027512-52-2 

Description

Learn about fluorous affinity purification of oligonucleotides here or download a brief overview here.
Download a Product Information sheet for FL 1400. If a short hydroxyalkyl group is tolerated at the 5' terminus, FDMT-On Purification Modifier is an effective way to enable fluorous purification in a variety of applications without having to purchase a different fluorous amidite for each different 5' nucleobase. This phosphoramidite installs a fluorous dimethoxytrityl group via a short tether. After fluorous affinity purification and detritylation, a 3-hydroxy-1-methylpropyl group remains. FDMT-On Purification Modifier couples with greater than 95% efficiency (typically >98%) under the standard conditions recommended for popular synthesizers. If a 15 minute coupling is used instead, the efficiency rises to typically >99%. Please note that while this reagent is freely soluble in acetonitrile, it is somewhat slow to dissolve. Allow 30 minutes with occasional swirling for complete dissolution. After cleavage from the support and nucleobase deprotection using standard techniques, the fluorous-tagged oligonucleotide is purified using a Fluoro-Pak Column (FP 7210 or FP 7220) and Loading Buffer (LB 7100). See "User Guide: Fluorous Purification of Oligonucleotides", which is included in with your purchase or may be downloaded. As usual, ammonia removal is not required, and detritylated oligonucleotides are obtained with high recovery, free from failure sequences.

Notes

Enables FDMT-on fluorous affinity purification without having to purchase individual FDMT-bearing nucleoside phosphoramidites.

Alternate Name(s):

1-O-[(Diisopropylamino)(2-cyanoethoxy)phosphino]-3-O-[4,4'-dimethoxy-4'-(1H,1H,2H,2H-perfluorodecyl)trityl]-1,3-butanediol

FL 1500

FMMT-5'-Amino-modifier-C6 CEP

C45H51F17N3O3P 

1027512-64-6 

Description

For applications requiring the purification of 5'-amine-modified oligonucleotides, trityl-on purification is an option, typically involving a monomethoxytrityl (MMT) group. In cases where the separation of the trityl-on oligonucleotides from failure sequences and other non-trityl-bearing materials is more difficult, FMMT-5'-Amino-modifier-C6 CEP (FL 1500) is a useful option. It employs a fluorous version of the MMT group, i.e., a fluorous monomethoxytrityl (FMMT) group. This phosphoramidite behaves similarly to the MMT versions during synthesis, but the final FMMT-bearing oligonucleotide is more strongly retained on fluorous adsorbents or reversed-phase adsorbents, providing greater selectivity than observed for MMT-bearing oligos.

For more detail on the use of this product, download a Product Information Sheet here.

1. Pearson, W. H.; Berry, D. A.; Stoy, P.; Jung, K.-Y.; Sercel, A. D. J. Org. Chem. 2005, 70, 7114-7122.

Notes

Installs an amine at the 5'-terminus of oligonucleotides enabling fluorous affinity purification.1 Employs the fluorous monomethoxytrityl (FMMT) group rather than an MMT group, providing greater discrimination of full-length oligonucleotides over failure sequences.

FL 1600

Fluorous modifier CEP

C47H53F17N3O7P 

1027512-58-8 

Description

While many of Berry & Associates' fluorous products focus on the purification of oligonucleotides,1 fluorous tags have other potential applications in nucleic acid chemistry. Fluorous modifier CEP (FL 1600) is useful for placing a permanent fluorous tag internally or at the 5'-terminus of an oligonucleotide. In addition to providing a purification handle, fluorous modifications enable applications where fluorophilicity or high hydrophobicity are desired. For example, the presence of a fluorous tag in an oligonucleotide may allow its immobilization onto fluorous-coated glass slides.2,3 Alternatively, placing fluorous monomers at strategic sites in an oligonucleotide may allow intra- or intermolecular fluorous-fluorous interactions, enhancing the attraction between various regions of an oligonucleotide.

For more detail on the use of this product (coupling, cleavage, deprotection, HPLC analysis, and Fluoro-Pak purification), download a Product Information Sheet here.

1. Pearson, W. H.; Berry, D. A.; Stoy, P.; Jung, K.-Y.; Sercel, A. D. J. Org. Chem. 2005, 70, 7114-7122.

2. Fluorous Technologies, Inc., offers Fluorous Modified Glass Slides for the immobilization of fluorous-tagged molecules for microarray formation. The slides feature excellent spot morphology, high signal-to-noise ratios, low and uniform background fluorescence levels, and low non-specific binding, since the fluorous surface around the spot does not interact well with non-fluorous molecules. The ability to use the fluorous tag as both a purification handle and an immobilization handle is also an advantage. Further, the fluorous immobilization is potentially reversible.

3. (a) Pohl and co-workers detected carbohydrate-lectin interactions using fluorous modified slides bearing fluorous-tagged carbohydrates; see: Ko, K.-S.; Jaipuri, F. A.; Pohl, N. L. J. Am. Chem. Soc. 2005, 127, 13162-13163. (b) Spring and co-workers showed that fluorous-tagged small molecules could be immobilized on fluorous modified a glass surface and used to facilitate detection of protein-ligand binding interactions; see: Nicholson, R. L.; Ladlow, M. L.; Spring, D. R. Chem. Commun. 2007, 3906-3908. © Schreiber and co-workers employed fluorous-immobilized small-molecule arrays to screen for histone deacylase inhibitors; see: Vegas, A. J.; Bradner, J. E.; Tang, W.; McPherson, O. M.; Greenberg, E. F.; Koehler, A. N.; Schreiber, S. L. Angew. Chem. Int. Ed. 2007, 46, 7960-7964.

Notes

Fluorous Modifier CEP (FL 1600) is useful for placing a permanent fluorous tag internally or at the 5'-terminus of an oligonucleotide.

FL 1610

3'-Fluorous modifier CPG

N/A 

N/A 

Description

While many of Berry & Associates' fluorous products focus on the purification of oligonucleotides,1 fluorous tags have other potential applications in nucleic acid chemistry. 3'-Fluorous modifier CPG (FL 1610) is useful for placing a permanent fluorous tag at the 3'-terminus of an oligonucleotide. In addition to providing a purification handle, fluorous modifications enable applications where fluorophilicity or high hydrophobicity are desired. For example, the presence of a fluorous tag in an oligonucleotide may allow its immobilization onto fluorous-coated glass slides.2,3 Alternatively, placing fluorous monomers at strategic sites in an oligonucleotide may allow intra- or intermolecular fluorous-fluorous interactions, enhancing the attraction between various regions of an oligonucleotide.

For more detail on the use of this product (coupling, cleavage, deprotection, HPLC analysis, and Fluoro-Pak purification), download a Product Information Sheet here.

1. Pearson, W. H.; Berry, D. A.; Stoy, P.; Jung, K.-Y.; Sercel, A. D. J. Org. Chem. 2005, 70, 7114-7122.

2. Fluorous Technologies, Inc., offers Fluorous Modified Glass Slides for the immobilization of fluorous-tagged molecules for microarray formation. The slides feature excellent spot morphology, high signal-to-noise ratios, low and uniform background fluorescence levels, and low non-specific binding, since the fluorous surface around the spot does not interact well with non-fluorous molecules. The ability to use the fluorous tag as both a purification handle and an immobilization handle is also an advantage. Further, the fluorous immobilization is potentially reversible.

3. (a) Pohl and co-workers detected carbohydrate-lectin interactions using fluorous modified slides bearing fluorous-tagged carbohydrates; see: Ko, K.-S.; Jaipuri, F. A.; Pohl, N. L. J. Am. Chem. Soc. 2005, 127, 13162-13163. (b) Spring and co-workers showed that fluorous-tagged small molecules could be immobilized on fluorous modified a glass surface and used to facilitate detection of protein-ligand binding interactions; see: Nicholson, R. L.; Ladlow, M. L.; Spring, D. R. Chem. Commun. 2007, 3906-3908. © Schreiber and co-workers employed fluorous-immobilized small-molecule arrays to screen for histone deacylase inhibitors; see: Vegas, A. J.; Bradner, J. E.; Tang, W.; McPherson, O. M.; Greenberg, E. F.; Koehler, A. N.; Schreiber, S. L. Angew. Chem. Int. Ed. 2007, 46, 7960-7964.

Notes

3'-Fluorous Modifier CPG (FL 1610) is useful for placing a permanent fluorous tag at the 3'-terminus of an oligonucleotide.

FL 1620

Fluorous propanol CEP

C20H24F17N2O2P 

1036029-24-9 

Description

The simple fluorous phosphoramidite Fluorous propanol CEP does not have a DMT group and is installed permanently at the 5'-terminus of an oligonucleotide. This reagent serves two purposes: (1) it is a simple way to install a permanent fluorous tag, which may be useful for many purposes including immobilization of the oligonucleotide onto a fluorous glass slide, increasing the hydrophobicity of an oligonucleotide, and or as a mass tag; and (2) this phosphoramidite may be used in the capping steps of oligonucleotide synthesis. When used in the capping steps of oligonucleotide synthesis, the full-length oligonucleotide does not have a fluorous tag incorporated. The failure sequences, however, are capped with a fluorous handle and can easily be removed by capture on fluorous or RP media since fluorous-tagged oligonucleotides are retained strongly on fluorous or RP-HPLC adsorbents, more so than molecules that bear dyes or normal DMT groups.

For more information, download a Product Information sheet.

Learn about fluorous affinity purification of oligonucleotides here. Download a brief overview here.

Notes

A fluorous phosphoramidite that is installed permanently at the 5'-terminus of an oligonucleotide.

FL 1700

FDMT-5'-Fluorescein CEP

C77H79F17N3O14P 

1159976-41-6 

Description

The highly hydrophobic FDMT group dominates the adsorptive properties of the molecules, allowing separation from non-FDMT-bearing materials using HPLC (fluorous or RP) or cartridge-based methods (FluoroPak or RP columns). The FDMT group can be removed using the same methods that are used with DMT groups, and on-column detritylation may be employed.

For more information, download a Product Information sheet.

Learn about fluorous affinity purification of oligonucleotides here.

Download a brief overview here.

Notes

Useful for the installation of fluorescein as well as a fluorous tag for greater affinity for a hydrophobic adsorbent improving purification.

FL 1710

Fluorous 5'-fluorescein CEP

C53H55F17N3O10P 

1026807-03-3 

Description

Dyes such as fluorescein are often introduced into oligonucleotides using a non-DMT-bearing phosphoramidite such as 5'-Fluorescein CEP ("6-FAM", BA 0054), which places a 6-carboxyfluorescein residue at the 5'-terminus and does not allow further extension. We now offer Fluorous 5'-fluorescein CEP ("Fluorous 6-FAM", FL 1710), where a permanently-attached fluorous tail is present, allowing the isolation of all oligonucleotides that bear a fluorescein moiety using fluorous or reversed-phase adsorbents. The fluorous tail may also enhance contact quenching with hydrophobic quenchers, especially those that have fluorous tails.

For more information, download a Product Information sheet.

Learn about fluorous affinity purification of oligonucleotides here. Download a brief overview here.

Notes

Useful for the introduction of fluorescein and a permanently-attached fluorous tail which allows the isolation of all oligonucleotides that bear the fluorescein moiety.

FL 1800

Fluorous 3'-dabcyl CPG

N/A 

N/A 

Description

Quenchers of fluorescence are often installed into an oligonucleotide probe using 3'-Dabcyl CPG. We now offer Fluorous 3'-dabcyl CPG, which bears a permanent fluorous tail, rendering all quencher-labeled oligonucleotides highly hydrophobic. The fluorous group dominates the adsorption characteristics of the oligonucleotide, causing it to be highly retained on RP and fluorous adsorbents. Whereas a probe bearing a normal 3'-dabcyl and a DMT-bearing 5'-(6-FAM) (from Fluorescein II CEP, BA 0253) was not well separated from dabcyl-only and FAM-only materials, the same probe bearing a fluorous 3'-dabcyl (from Fluorous 3'-dabcyl CPG, FL 1800) and a DMT-bearing 5'-(6-FAM) was well separated from non-fluorous materials. Oligos bearing only a fluorous dabcyl (but no fluorescein) were also strongly retained. Thus, with a 3'-fluorous dabcyl-labeled oligonucleotide, all highly-retained oligos bear dabcyls and are thus dark; i.e., oligos bearing only a fluorophore are easily separated.

Learn about fluorous affinity purification of oligonucleotides here. Download a brief overview here.

Notes

Fluorous 3'-Dabcyl CPG, which bears a permanent fluorous tail, renders all quencher-labeled oligonucleotides highly hydrophobic.

FP 7210

Fluoro-Pak™ Columns

N/A 

N/A 

Description

Fluoro-PakTM Columns: Each column contains 75 mg of adsorbent, and may be used for up to 0.2 micromole purifications.

For more information on fluorous purification, see this link. Download a brief overview (pdf) of fluorous affinity purification here. See product FP 7210-A if a free Luer adaptor is needed. Please note: Fluoro-Pak™ columns are designed to be used with fluorous-tagged oligonucleotides but are also useful for normal DMT-on purifications.

Notes

Used for the fluorous affinity purification of oligonucleotides.

FP 7220

Fluoro-Pak™ II Columns

N/A 

N/A 

Description

Fluoro-PakTM II Columns: Each column contains 150 mg of adsorbent, and may be used for up to 1 micromole purifications.

Learn more about fluorous affinity purification of oligonucleotides here. Download a brief overview (pdf) here. See product FP 7220-A if a free Luer adaptor is needed. Please note: Fluoro-Pak™ columns are designed to be used with fluorous-tagged oligonucleotides but are also useful for normal DMT-on purifications.

Notes

Used for the fluorous affinity purification of oligonucleotides.

FT 6200

5-Carboxytetramethylrhodamine

C25H22N2O5 

91809-66-4 or 150322-05-7 

Alternate Name(s):

5-Carboxy TAMRA

FT 6210

6-Carboxytetramethylrhodamine

C25H22N2O5 

91809-67-5 or 150322-06-8 

Alternate Name(s):

6-Carboxy TAMRA

FT 6220

5-Carboxytetramethylrhodamine N-
Hydroxysuccinimide Ester

C29H25N3O7 

150810-68-7 or 321862-17-3 

Alternate Name(s):

5-Carboxy TAMRA N-hydroxysuccinimide ester

FT 6230

6-Carboxytetramethylrhodamine N-
Hydroxysuccinimide Ester

C29H25N3O7 

150810-69-8 

Alternate Name(s):

6-Carboxy TAMRA N-hydroxysuccinimide ester

FT 6240

6-Carboxy-TAMRA TEG azide

C33H38N6O7 

None Assigned 



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